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THUNDER™ Phospho-STAT1 (Y701) TR-FRET Cell Signaling Assay Kit

2021-06-25

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT1 (Y701) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: the Phospho-STAT1 (Y701) Assay Kit uses a new antibody pair that significantly improves the assay performance.

Product citation: PubMed 34570089

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Data show the superior performance of the new Phospho-STAT1 (Y701) Assay compared to the previous version of the assay.
Stimulation of Phospho-STAT1 in HeLa cells
Data show that treatment of B cells with IFNα2b stimulates phosphorylation of STAT1 at Y701.
Inhibition of Phospho-STAT1 in HeLa cells
Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT1 at Y701 by IFNα2b.
Z'-factor of Phospho-STAT1 in HeLa cells
The Z’-factor value of 0.91 indicates that the assay in HeLa cells is robust and suitable for HTS.
Stimulation of Phospho-STAT1 in B cells
Data show that treatment of B cells with IFNα2b stimulates phosphorylation of STAT1 at Y701.
Inhibition of Phospho-STAT1 in B cells
Data show that treatment of B cells with JAK Inhibitor 1 inhibits phosphorylation of STAT1 at Y701 by IFNα2b.
Stimulation of Phospho-STAT1 in NIH/3T3 cells
Data show that treatment of NIH/3T3 cells with reference cytokines stimulates phosphorylation of STAT1 at Y701.