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THUNDER™ Phospho-STAT1 (Y701) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT1 (Y701) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: the Phospho-STAT1 (Y701) Assay Kit uses a new antibody pair that significantly improves the assay performance.

Product citation: PubMed 34570089

BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Data show the superior performance of the new Phospho-STAT1 (Y701) Assay compared to the previous version of the assay
Data show that treatment of B cells with IFNα2b stimulates phosphorylation of STAT1 at Y701.
Data show that treatment of B cells with JAK Inhibitor 1 inhibits phosphorylation of STAT1 at Y701 by IFNα2b.
The Z’-factor value of 0.85 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.