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THUNDER™ Phospho-AKT pan (T308) + Total AKT pan TR-FRET Cell Signaling Assay Kit

2019-12-12

$1,195.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT pan (T308) and Total AKT pan in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total AKT pan proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.
Stimulation of Phospho-AKT pan (T308) in NIH3T3 cells
Treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at T308 but does not affect the levels of total AKT pan.
Inhibition of Phospho-AKT pan (T308) in NIH3T3 cells
Treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at T308 by PDGF-AA, but does not affect the levels of total AKT pan.
NIH3T3 control lysate titration (QC test) Phospho-AKT pan (T308)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
NIH3T3 control lysate titration (QC test) Total AKT pan
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.