Data show that treatment of MCF7 cells with insulin stimulates phosphorylation of RPS6 at S235/S236
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of RPS6 at S235/S236 by insulin
The Z’-factor value of 0.52 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
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