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THUNDER™ Phospho-TBK1 (S172) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-TBK1/NAK (S172) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Data show that treatment of MCF7 cells with Calyculin A stimulates phosphorylation of TBK1/NAK at S172.
Data show that treatment of MCF7 cells with BX-795 inhibits phosphorylation of TBK1/NAK at S172 by Calyculin A.
The Z’-factor value of 0.70 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Data show the robustness of the assay following overnight incubation.