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THUNDER™ Phospho-EGFR (Y1068) + Total EGFR TR-FRET Cell Signaling Assay Kit

2019-12-12

$1,195.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EGFR (Y1068) and Total EGFR in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total EGFR proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.
Stimulation of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of A431 cells with EGF stimulates phosphorylation of EGFR at Y1068 but does not have a major effect on the levels of total EGFR.
Inhibition of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of A431 cells with Cetuximab inhibits phosphorylation of EGFR at Y1068 by EGF but does not have a major effect on the levels of total EGFR.
A431 control lysate titration (QC Test)Phospho-EGFR (Y1068)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
A431 control lysate titration(QC Test) Total EGFR
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.