Data show that treatment of HEK293 cells with Anisomycin stimulates phosphorylation of JNK1/2/3 at T183/Y185.
Data show that treatment of HEK293 cells with BX 795 inhibits phosphorylation of JNK1/2/3 at T183/Y185 by Anisomycin.
The Z’-factor value of 0.82 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
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