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THUNDER™ Phospho-BTK (Y223) TR-FRET Cell Signaling Assay Kit

2021-06-23

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BTK (Y223) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-BTK (Y223) in Raji cells
Data show that treatment of Raji cells with Pervanadate stimulates phosphorylation of BTK at Y223
Inhibition of Phospho-BTK (Y223) in Raji cells
Data show that treatment of Raji cells with Ibrutinib inhibits phosphorylation of BTK at Y223 by Pervanadate
Z'-factor determination in Raji cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS
Phospho-BTK (Y223) Control lysate titration
Data show a linear relationship between lysate dilutions and TR-FRET ratio values