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Phospho-ERK1/2 (T202/Y204)

2019-12-13

$335.00$1,057.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204)

N/A

Category:
Clear
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information

Size

100 points, 500 points

Stimulation of PHOSPHO-ERK1-2 (T202-Y204) in  HEK293 cells
Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204.
Inhibition of Phospho-ERK1-2 (T202-Y204) in HEK293 cells
Data show that treatment of HEK293 cells with Erlotinib inhibits phosphorylation of ERK1/2 at T202/Y204 by EGF.
Z'-factor determination (QC Test) HEK293 cells
The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.