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THUNDER™ Total NF-kB TR-FRET Cell Signaling Assay Kit

2019-12-13

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total NF-κB (both phosphorylated and non-phosphorylated NF-κB) in cell lysates. This kit can be used to normalize data obtained with the Phospho-NF-κB (S536) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Stimulation of Phospho-NF-kB (S536) in HeLa cells
Data show that treatment of HeLa cells with TNFα stimulates phosphorylation of NF-kB at S536, but does not affect the levels of total NF-kB
Inhibition of Phospho-NF-kB (S536) in HeLa cells
Data show that treatment of HeLa cells with IKK-16 inhibits phosphorylation of NF-kB at S536 by TNFα, but does not affect the levels of total NF-kB
HeLa control lyste titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values