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THUNDER™ cAMP TR-FRET Assay Kit

2021-11-22

$558.00$3,533.00

Homogeneous competitive TR-FRET immunoassay for quantitative measurement of cAMP in cell lysates. The kit is compatible with both suspension and adherent cells and is ideal for the pharmacological characterization and high-throughput screening of Gs- and Gi-coupled GPCRs. A detailed assay protocol and full assay validation can be found in the Technical Data Sheet. BEST SELLER

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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1000 points, 5000 points, 20000 points

Assay principle: Free cAMP produced by cells competes with the Europium labeled streptavidin/biotin-cAMP tracer complex for binding to a monoclonal cAMP antibody labeled with the far-red fluorophore. The specific TR-FRET signal is therefore inversely proportional to the concentration of cAMP in the lysate.
THUNDER vs Competitor-cAMP standard curve
The THUNDER™ cAMP assay exhibits a comparable performance to that of a competing TR-FRET assay.
The THUNDER™ cAMP assay workflow consists of only two steps.