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THUNDER™ cAMP TR-FRET Assay Kit

2021-11-22

$485.00$3,100.00

Homogeneous competitive TR-FRET immunoassay for quantitative measurement of cAMP in cell lysates. The kit is compatible with both suspension and adherent cells and is ideal for the pharmacological characterization and screening of Gs- and Gi-coupled GPCRs. A detailed assay protocol and full assay validation can be found in the Technical Data Sheet.

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Clear
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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1000 points, 5000 points, 20000 points

Assay principle: Free cAMP produced by cells competes with the Europium labeled streptavidin/biotin-cAMP tracer complex for binding to a monoclonal cAMP antibody labeled with the far-red fluorophore. The specific TR-FRET signal is therefore inversely proportional to the concentration of cAMP in the lysate.
THUNDER vs Competitor-cAMP standard curve
The THUNDER™ cAMP assay exhibits a comparable performance to that of a competing TR-FRET assay.
The THUNDER™ cAMP assay workflow consists of only two steps.