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THUNDER™
TR-FRET

CELL SIGNALING ASSAY KITS

THUNDER™
TR-FRET

BIOMARKER ASSAY KITS
Human IFNβ
Mouse IFNβ
Human IFNα
Human IFNγ
Mouse IFNγ
Human IL-1α
Mouse IL-1α
Human IL-1β
Mouse IL-1β
Human IL-2
Mouse IL-2
Human IL-6
Mouse IL-6
Human IL-8/CXCL8
Human IL-10
Human IL-11
Human IL-12/IL-23 p40
Human IL-12 p70
Human IL-17A
Human IL-18
Human Fibronectin
Human Insulin
Human CCL2/MCP1
Mouse CCL2/MCP1
Human CCL3/MIP1α
Human CCL4/MIP-1β
Human CXCL1/GROα
Human CXCL9/MIG
Human TNFα
Human MMP-3
Human MMP-7
Human PD-L1
Human TNFα
Mouse TNFα
Human GM-CSF
Human COL1A1
Human VEGF
Mouse VEGF
Human IgG (Fc)
Mouse IgG
Rabbit IgG (Fc)

THUNDER™
TR-FRET

cAMP ASSAY KITS
cAMP assay kit

THUNDER™
TR-FRET
TOOLBOX REAGENTS

EUROPIUM LABELED
Europium-labeled anti-6xHis
Europium-labeled anti-FLAG
Europium-labeled anti-GST
Europium-labeled anti-V5
Europium-labeled anti-human IgG
Europium-labeled anti-mouse IgG
Europium-labeled anti-rabbit IgG
Europium-labeled streptavidin
ACCEPTOR LABELED
Acceptor-labeled anti-6xHis
Acceptor-labeled anti-FLAG
Acceptor-labeled anti-GST
Acceptor-labeled anti-V5
Acceptor-labeled anti-human IgG
Acceptor-labeled anti-mouse IgG
Acceptor-labeled anti-rabbit IgG
Acceptor-labeled streptavidin

COMPLEMENTARY PRODUCTS

READER CONTROL
THUNDER™ Reader Control TR-FRET Kit
POSITIVE CONTROL LYSATES
THUNDER™ 4EBP1 Control lysate
THUNDER™ AKT pan Control lysate
THUNDER™ BAD Control lysate
THUNDER™ CREB Control lysate
THUNDER™ EGFR Control lysate
THUNDER™ eIF2α Control lysate
THUNDER™ eIF4E Control lysate
THUNDER™ ERK Control lysate
THUNDER™ GAPDH Control lysate
THUNDER™ GSK3β Control lysate
THUNDER™ INR Control lysate
THUNDER™ JNK Control lysate
THUNDER™ MEK1 Control lysate
THUNDER™ MET Control lysate
THUNDER™ NF-κB Control lysate
THUNDER™ p38 Control lysate
THUNDER™ p53 Control lysate
THUNDER™ P70S6 Control lysate
THUNDER™ RPS6 Control lysate
THUNDER™ SLP-76 Control lysate
THUNDER™ SMAD2 Control lysate
THUNDER™ SRC Control lysate
THUNDER™ STAT3 Control lysate
THUNDER™ STAT5 Control lysate
THUNDER™ STAT6 Control lysate
LYSIS BUFFERS
THUNDER™ Lysis Buffer 1 (5X)
THUNDER™ Lysis Buffer 2 (5X)
THUNDER™ Lysis Buffer 3 (5X)
THUNDER™ Lysis Buffer 4 (5X)
THUNDER™ Lysis Buffer 5 (5X)
THUNDER™ TR-FRET cAMP Detection Buffer (5X)
DETECTION BUFFER
THUNDER™ TR-FRET Assay Buffer 1 (5X)
THUNDER™ TR-FRET Assay Buffer 2 (5X)
THUNDER™ TR-FRET Assay Buffer 3 (5X)
THUNDER™ TR-FRET Assay Buffer 4 (5X)
THUNDER™ TR-FRET cAMP Detection Buffer (5X)
THUNDER™ TR-FRET Detection Buffer (10X)
PHOSPHATASE INHIBITOR COCKTAIL
THUNDER™ Phosphatase Inhibitor Cocktail (100X)

THUNDER™ cAMP TR-FRET Assay Kit

2021-11-22
COMPLEMENTARY REAGENTS THUNDER™ TR-FRET cAMP Detection Buffer (5X)
View technical data sheet

$521.00 – $3,333.00

Homogeneous competitive TR-FRET immunoassay for quantitative measurement of cAMP in cell lysates. The kit is compatible with both suspension and adherent cells and is ideal for the pharmacological characterization and screening of Gs- and Gi-coupled GPCRs. A detailed assay protocol and full assay validation can be found in the Technical Data Sheet.

N/A

Category:
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information

Size

1000 points, 5000 points, 20000 points
Assay principle: Free cAMP produced by cells competes with the Europium labeled streptavidin/biotin-cAMP tracer complex for binding to a monoclonal cAMP antibody labeled with the far-red fluorophore. The specific TR-FRET signal is therefore inversely proportional to the concentration of cAMP in the lysate.
THUNDER vs Competitor-cAMP standard curve
The THUNDER™ cAMP assay exhibits a comparable performance to that of a competing TR-FRET assay.
The THUNDER™ cAMP assay workflow consists of only two steps.
Bioauxilium specializes in the design, development and manufacturing of ready-to-use TR-FRET assay kits that simplify laboratory workflow and accelerate drug discovery and life sciences research.
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