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THUNDER™ Phospho-eIF2α (S51) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EIF2α (S51) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Stimulation of Phospho-eIF2α (S51) in Hela cells
Data show that treatment of HeLa cells with Calyculin A stimulates phosphorylation of eIF2α at S51.
Z'-factor determination in Hela cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
Hela Control cell lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.