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THUNDER™ Phospho-SYK (Y525/Y526) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SYK (Y525/Y526) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information


100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-SYK (Y525/Y526) in Raji cells
Data show that treatment of Raji cells with pervanadate stimulates phosphorylation of SYK at Y525/Y526
Inhibition of Phospho-SYK (Y525/Y526) in U-937 cells
Data show that treatment of U-937 cells with BIIB-057 inhibits phosphorylation of SYK at Y525/Y526 by pervanadate
Z'-factor determination in Raji cells
The Z’-factor value of 0.82 indicates that the assay is robust and suitable for HTS
U-937 control lysate titration (QC Test)
Data show a linear relationship between lysates pre-diluted at 1:4 or more and TR-FRET ratio values