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THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 (Y705) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.


Note: the Phospho-STAT3 (Y705) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available.

Product citation: PubMed 34570089


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information


100 points, 500 points, 2500 points, 5000 points, 10000 points

New versus Old Phospho-STAT3 Assay Kit
Data show the superior performance of the new Phospho-STAT3 (Y705) Assay compared to the previous version of the assay
Stimulation of Phospho-STAT3 (Y705) in HeLa cells
Data show that treatment of HeLa cells with IFNα stimulates phosphorylation of STAT3 at Y705
Inhibition of Phospho-STAT3 (Y705) in HeLa cells
Data show that treatment of HeLa cells with JAK Innibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα
Z'-factor determination in HeLa cells
The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
Phospho-STAT3 benchmarking-THUNDER
The THUNDER assay shows the highest S/B ratios between 1 and 4 hours of incubation
Phospho-STAT3 benchmarking-Company A
Company A assay shows a comparable S/B ratio to the THUNDER assay only after overnight incubation
Phospho-STAT3 benchmarking-Company B
Company B assay shows the lowest S/B ratios at all incubation times