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THUNDER™ Total GAPDH TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Total GAPDH in cell lysates. Measurement of GAPDH may be a useful internal control for monitoring total protein levels when measuring changes in specific phosphorylated and/or total proteins. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: owing to the high sensitivity of this kit, the lysates need to be diluted 10-fold in Lysis Buffer 3 or 4 before testing. This kit is only compatible with cell lysates generated with the Lysis Buffer 3 or Lysis Buffer 4.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information


100 points, 500 points, 2500 points, 5000 points, 10000 points

Total GAPDH Adherent cells
Total GAPDH Suspension cells
Detection GAPDH by Western-blot
A specific band was detected for GAPDH at approximately 37 kDa.