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THUNDER™ Phospho-p53 (S15) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p53 (S15) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information


100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-P53 (S15) in HEK293 cells
Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at Ser15
Z'-factor determination in HEK293 cells
The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TRFRET ratio values