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THUNDER™ Phospho-SLP-76 (S376) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SLP-76 (S376) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: the Phospho-SLP-76 (S376) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay performs comparably to an alternative TR-FRET assay available.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information


100 points, 500 points, 2500 points, 5000 points, 10000 points

Benchmarking of THUNDER Phospho-SLP-76 (S376) vs Company A
The THUNDER assay shows a higher S/B ratio than Company A assay
Benchmarking of THUNDER Phospho-SLP-76 (S376) vs Company B
The THUNDER and Company B assays show comparable S/B ratios
Stimulation of Phospho-SLP-76 (S376) in Jurkat cells
Data show that treatment of Jurkat cells with H2O2 stimulates phosphorylation of SLP-76 at S376
Z'-factor determination in Jurkat cells
The Z’-factor value of 0.92 indicates that the assay is robust and suitable for HTS
Jurkat control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
All-in-one-well Phopsho-SLP-76 (S376) assay
Data show that stimulation of phospho-SLP-76 (S376) by H2O2 in Jurkat cells can be detected using the 1-plate assay protocol in both LV 384 and HA 96-well plates