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THUNDER™ Phospho-SMAD3 (S423/S425) TR-FRET Cell Signaling Assay Kit

2025-06-06

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD3 (S423/S425) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Inhibition of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with SB 431542 inhibits phosphorylation of SMAD3 at S423/S425 by TGFβ.
Z'-factor determination in HeLa cells
The Z’-factor value of 0.65 indicates that the assay is robust and suitable for HTS
Kit benchmarking Phospho-SMAD2
The THUNDER assay shows a comparable S/B ratio and EC50 value to the competitor TR-FRET assay after 2h of incubation (80,000 cells/well).
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values