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THUNDER™ Phospho-MEK1 (S218/S222) + Total MEK1 TR-FRET Cell Signaling Assay Kit

2019-12-13

$1,195.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-MEK1 (S218/S222) and Total MEK1 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total MEK1 proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
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Stimulation of Phospho-MEK1 (S218-S222) in HeLa cells
Data show that treatment of HeLa cells with PMA stimulates phosphorylation of MEK1 at S218/S222, but does not affect the levels of total MEK1.
Inhibition of Phospho-MEK1 (S218-S222) in HeLa cells
Data show that treatment of HeLa cells with L779,450 inhibits phosphorylation of MEK1 at S218/S222 by PMA, but does not affect the levels of total MEK1.
HeLa control lysate titration (QC Test) Phospho-MEK1 (S218-S222)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
HeLa control lysate titration (QC Test) Total MEK1
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.