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TR-FRET CELL

SIGNALING ASSAY KITS

COMPLEMENTARY PRODUCTS

POSITIVE CONTROL LYSATES
4EBP1 Control lysate
AKT pan Control lysate
BAD Control lysate
CREB Control lysate
EGFR Control lysate
eIF2α Control lysate
eIF4E Control lysate
ERK Control lysate
GAPDH Control lysate
GSK3β Control lysate
INR Control lysate
JNK Control lysate
MEK1 Control lysate
MET Control lysate
NF-κB Control lysate
p38 Control lysate
p53 Control lysate
P70S6 Control lysate
RPS6 Control lysate
SLP-76 Control lysate
SMAD2 Control lysate
SRC Control lysate
STAT3 Control lysate
STAT5 Control lysate
STAT6 Control lysate
LYSIS BUFFERS
Lysis Buffer 1 (5X), 10 mL
Lysis Buffer 2 (5X), 10 mL
Lysis Buffer 3 (5X), 10 mL
Lysis Buffer 4 (5X), 10 mL
Lysis Buffer 5 (5X), 10 mL
Lysis Buffer 6 (5X), 10 mL
DETECTION BUFFER
TR-FRET Detection Buffer (10X), 2 mL

Phospho-MEK1 (S218/S222) + Total MEK1

2019-12-13
COMPLEMENTARY REAGENTS MEK1 Control lysate Lysis Buffer 3 (5X), 10 mL TR-FRET Detection Buffer (10X), 2 mL
View technical data sheet

$1,135.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-MEK1 (S218/S222) and Total MEK1

N/A

Category:
Clear
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Stimulation of Phospho-MEK1 (S218-S222) in HeLa cells
Data show that treatment of HeLa cells with PMA stimulates phosphorylation of MEK1 at S218/S222, but does not affect the levels of total MEK1.
Inhibition of Phospho-MEK1 (S218-S222) in HeLa cells
Data show that treatment of HeLa cells with L779,450 inhibits phosphorylation of MEK1 at S218/S222 by PMA, but does not affect the levels of total MEK1.
HeLa control lysate titration (QC Test) Phospho-MEK1 (S218-S222)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
HeLa control lysate titration (QC Test) Total MEK1
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Bioauxilium specializes in the design, development and manufacturing of ready-to-use TR-FRET assay kits that simplify laboratory workflow and accelerate drug discovery and life sciences research.
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