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THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-4EBP1 (T37/T46) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Inhibition of Phospho 4EBP1 (T37-T46)in A431 cells
Treatment of A431 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46.
Inhibition of Phospho 4EBP1 (T37-T46) in HEK293 cells
Treatment of HEK293 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46
Z-factor determination in A431 cells
Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS
A431 control lysate titration (QC test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values