Treatment of A431 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46.
Treatment of HEK293 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46
Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
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