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THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-4EBP1 (T37/T46) in cell lysates.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Inhibition of Phospho 4EBP1 (T37-T46)in A431 cells
Treatment of A431 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46.
Inhibition of Phospho 4EBP1 (T37-T46) in HEK293 cells
Treatment of HEK293 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46
Z-factor determination in A431 cells
Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS
A431 control lysate titration (QC test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values