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THUNDER™ Total SLP-76 TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Total SLP-76 (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-SLP-76 (S376) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Stimulation of Phospho-SLP-76 (S376) in Jurkat cells
Data show that treatment of Jurkat cells with H2O2 stimulates phosphorylation of SLP-76 at S376 but does not affect the levels of total SLP-76
Jurkat control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values