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THUNDER™ Total SMAD3 TR-FRET Cell Signaling Assay Kit

2025-06-13

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total SMAD3 (both phosphorylated and non-phosphorylated SMAD3) in cell lysates. This kit can be used to normalize data obtained with the Phospho-SMAD3 (S423/S425) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with TGFβ stimulates phosphorylation of SMAD3 at S423/S425, but does not affect the levels of total SMAD3.
Inhibition of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with SB 431542 inhibits phosphorylation of SMAD3 at S423/S425 by TGFβ, but does not affect the levels of total SMAD3.
A549 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values