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THUNDER™ Phospho-LCK (Y394) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-LCK (Y394) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information


100 points, 500 points, 2500 points, 5000 points, 10000 points

Data show that treatment of Jurkat cells with Pervanadate stimulates phosphorylation of LCK at Y394.
The Z’-factor value of 0.72 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.