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THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit

2019-12-12

$355.00$7,315.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-eIF4E (S209) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

N/A

Category:
Clear
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Inhibition of Phospho-eIF4E (S209) in HEK293 cells
Data show that treatment of HEK293 cells with CGP 57380 inhibits phosphorylation of eIF4E at S209 by EGF.
Inhibition of Phospho-eIF4E (S209) in HeLa cells
Data show that treatment of HeLa cells with CGP 57380 or PP242 inhibits phosphorylation of eIF4E at S209 by EGF.
Z'-factor determination in HEK293 cells
The Z’-factor value of 0.68 indicates that the assay is robust and suitable for HTS.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.