Name: THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit
SKU: 227
Availability: InStock

THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit

2019-12-12

View technical data sheet View generic user manual

$382.00 – $7,842.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-eIF4E (S209) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

N/A

Category: elF4E

Size

Clear

BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Inhibition of Phospho-eIF4E (S209) in HEK293 cells

Data show that treatment of HEK293 cells with CGP 57380 inhibits phosphorylation of eIF4E at S209 by EGF.

Inhibition of Phospho-eIF4E (S209) in HeLa cells

Data show that treatment of HeLa cells with CGP 57380 or PP242 inhibits phosphorylation of eIF4E at S209 by EGF.

The Z’-factor value of 0.68 indicates that the assay is robust and suitable for HTS.

HEK293 control lysate titration (QC Test)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-eIF4E (S209) TR-FRET Cell Signaling Assay Kit

Additional information

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TOOLBOX REAGENTS