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THUNDER™ Total RPS6 TR-FRET Cell Signaling Assay Kit

2019-12-13

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total Ribosomal Protein S6 (both phosphorylated and non-phosphorylated RPS6) in cell lysates. This kit can be used to normalize data obtained with the Phospho-RPS6 (S235/S236) kit and the Phospho-RPS6 (S240/S244) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Stimulation of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with insulin stimulates phosphorylation of RPS6 at S235/S236, but does not affect the levels of total RPS6
Inhibition of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of RPS6 at S235/S236 by insulin, but does not affect the levels of total RPS6
MCF7 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values