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THUNDER™ Total AKT pan TR-FRET Cell Signaling Assay Kit

2019-12-12

$355.00$7,315.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total AKT pan (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-AKT pan (S473) kit. and the Phospho-AKT pan (T308) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Clear
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Stimulation of Phospho-AKT pan (S473) in NIH3T3 cells
Data show that treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.
Inhibition of Phospho-AKT pan (S473) in NIH3T cells
Data show that treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473 by PDGF-AA but does not affect the levels of total AKT pan.
NIH3T3 control lysate titration (QC test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.