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THUNDER™ Total STAT6 TR-FRET Cell Signaling Assay Kit

2019-12-13

$355.00$7,315.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total STAT6 (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-STAT6 (Y641) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
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Stimulation of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with IL-4 stimulates phosphorylation of STAT6 at Y641, but does not affect the levels of total STAT6.
Inhibition of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4, but does not affect the levels of total STAT6.
HeLa control lysate titration (QC test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.