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THUNDER™ Phospho-p70 S6K (T389) + Total p70 S6K TR-FRET Cell Signaling Assay Kit

2019-12-13

$1,195.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p70 S6K (T389) and Total p70 S6K in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total p70 S6K proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.
Stimulation of Phospho-P70 S6K (T389) in MCF7 cells
. Data show that treatment of MCF7 cells with Insulin stimulates phosphorylation of p70 S6K at T389, but does not affect the levels of total p70 S6K
Stimulation of Phospho-P70 S6K (T389) in MCF7 cells
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of p70 S6K at T202/Y204 by insulin, but does not affect the levels of total p70 S6K
MCF7 control lysate titration (QC Test) Phospho-P70 S6K (T389)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
MCF7 control lysate titration (QC Test) total P70 S6K
Data show a linear relationship between lysate dilutions and TR-FRET ratio values