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Phospho-RPS6 (S235/S236) + Total RPS6 TR-FRET Cell Signaling Assay Kit

2019-12-13

$1,195.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Ribosomal Protein S6 (S235/S236) and Total Ribosomal Protein S6 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total Ribosomal Protein S6 proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
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Stimulation of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with insulin stimulates phosphorylation of RPS6 at S235/S236, but does not affect the levels of total RPS6
Inhibition of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of RPS6 at S235/S236 by insulin, but does not affect the levels of total RPS6
MCF7 control lysate titration (QC Test) Phospho-RPS6 (S235/S236)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
MCF7 control lysate titration (QC Test) total RPS6
Data show a linear relationship between lysate dilutions and TR-FRET ratio values