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THUNDER™ Phospho-EGFR (Y1068) TR-FRET Cell Signaling Assay Kit

2019-12-12

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EGFR (Y1068) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

N/A

Category:
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of A431 cells with EGF stimulates phosphorylation of EGFR at Y1068.
Inhibition of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of A431 cells with Cetuximab inhibits phosphorylation of EGFR at Y1068 by EGF.
Z'-factor determination in A431 cells
The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS.
A431 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.