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THUNDER™ Total SYK TR-FRET Cell Signaling Assay Kit

2023-08-18

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total SYK in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-SYK (Y525/Y526) in Raji cells
Data show that treatment of Raji cells with pervanadate stimulates phosphorylation of SYK at Y525/Y526 but does not affect the levels of Total SYK
Inhibition of Phospho-SYK (Y525/Y526) in U-937 cells
Data show that treatment of U-937 cells with BIIB-057 inhibits phosphorylation of SYK at Y525/Y526 by pervanadate but does not affect the levels of Total SYK
U-937 control lysate titration (QC Test) - Total SYK
Data show a linear relationship between lysate dilutions and TR-FRET ratio values