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THUNDER™ Total p53 TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Total p53 in cell lysates. This kit can be used to normalize data obtained with the Phospho-p53 (S15) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Stimulation of Phospho-P53 (S15) in HEK293 cells
Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at S15, but does not affect the levels of total p53
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values