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THUNDER™ Total eIF4E TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Total eIF4E (both phosphorylated and non-phosphorylated eIF4E) in cell lysates. This kit can be used to normalize data obtained with the Phospho-eIF4E (S209) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Inhibition of Phospho-eIF4E (S209) in HEK293 cells
Data show that treatment of HEK293 cells with CGP 57380 inhibits phosphorylation of eIF4E at S209 by EGF, but does not affect the levels of total eIF4E.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.