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THUNDER™ Total ERK1/2 TR-FRET Cell Signaling Assay Kit

2019-12-12

$355.00$7,315.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total ERK1/2 (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-ERK1/2 (T202/Y204) kit and the Extreme Phospho-ERK1/2 (T202/Y204) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Stimulation of PHOSPHO-ERK1-2(T202-Y204) in HEK293 cells
Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204, but does not affect the levels of total ERK1/2.
Inhibition of PHOSPHO-ERK1-2 (T202-Y204) in HEK293 cells
Data show that treatment of HEK293 cells with Erlotinib inhibits phosphorylation of ERK1/2 at T202/Y204 by EGF, but does not affect the levels of total ERK1/2.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.