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THUNDER™ Phospho-CREB (S133) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-CREB (S133) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho CREB (S133) in NIH3T3 cells
Data show that treatment of NIH3T3 cells with Forskolin stimulates phosphorylation of CREB at S133.
Inhibition of Phospho CREB (S133) in A431 cells
Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of CREB at S133 by EGF.
Z'-factor determination in A431 cells
The Z’-factor value of 0.61 indicates that the assay is robust and suitable for HTS.
B lymphocyte control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.