Data show that treatment of NIH3T3 cells with Forskolin stimulates phosphorylation of CREB at S133.
Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of CREB at S133 by EGF.
The Z’-factor value of 0.61 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
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