Data show that treatment of A431 or HeLa cells with HGF stimulates phosphorylation of c-Met at Y1234/Y1235.
Data show that treatment of HeLa cells with PHA-665752 inhibits phosphorylation of c-Met at Y1234/Y1235 by HGF.
The Z’-factor value of 0.7 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
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