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THUNDER™ Phospho-GSK3β (S9) + Total GSK3β TR-FRET Cell Signaling Assay Kit

2019-12-13

$1,195.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-GSK3β (S9) and Total GSK3β in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total GSK3β proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.
Stimulation of Phospho-GSK3β (S9) in MCF7 cells
Data show that treatment of MCF7 cells with EGF stimulates phosphorylation of GSK3β at S9 but does not affect the levels of total GSK3β.
Inhibition of Phospho-GSK3β (S9) in MCF7 cells
Data show that treatment of MCF7 cells with UCN-01 inhibits phosphorylation of GSK3β at S9 by Insulin, but does not affect the levels of total GSK3β.
MCF7 control-lysate titration (QC Test) Phospho-GSK3β (S9)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
MCF7 control lysate titration (QC Test) TOTAL GSK3β
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.