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THUNDER™
TR-FRET

CELL SIGNALING ASSAY KITS

THUNDER™
TR-FRET

BIOMARKER ASSAY KITS
Human IFNβ
Mouse IFNβ
Human IFNα
Human IFNγ
Mouse IFNγ
Human IL-1α
Mouse IL-1α
Human IL-1β
Mouse IL-1β
Human IL-2
Human IL-4
Mouse IL-2
Human IL-6
Mouse IL-6
Human IL-8/CXCL8
Human IL-10
Human IL-11
Human IL-12/IL-23 p40
Human IL-12 p70
Human IL-17A
Human IL-18
Human Fibronectin
Human Granzyme B
Human Insulin
Human CCL2/MCP1
Mouse CCL2/MCP1
Human CCL3/MIP1α
Human CCL4/MIP-1β
Human CCL17/TARC
Mouse CCL17/TARC
Human CXCL1/GROα
Human CXCL9/MIG
Human TNFα
Human MMP-3
Human MMP-7
Human PD-L1
Human TNFα
Mouse TNFα
Human GM-CSF
Human COL1A1
Human VEGF
Mouse VEGF
Human IgG (Fc)
Mouse IgG
Rabbit IgG (Fc)
CHO HCP

THUNDER™
TR-FRET

cAMP ASSAY KITS
cAMP assay kit

THUNDER™
TR-FRET
TOOLBOX REAGENTS

EUROPIUM LABELED
Europium-labeled anti-6xHis
Europium-labeled anti-FLAG
Europium-labeled anti-GST
Europium-labeled anti-V5
Europium-labeled anti-human IgG
Europium-labeled anti-mouse IgG
Europium-labeled anti-rabbit IgG
Europium-labeled streptavidin
ACCEPTOR LABELED
Acceptor-labeled anti-6xHis
Acceptor-labeled anti-FLAG
Acceptor-labeled anti-GST
Acceptor-labeled anti-V5
Acceptor-labeled anti-human IgG
Acceptor-labeled anti-mouse IgG
Acceptor-labeled anti-rabbit IgG
Acceptor-labeled streptavidin

COMPLEMENTARY PRODUCTS

READER CONTROL
THUNDER™ Reader Control TR-FRET Kit
POSITIVE CONTROL LYSATES
THUNDER™ 4EBP1 Control lysate
THUNDER™ AKT pan Control lysate
THUNDER™ BAD Control lysate
THUNDER™ CREB Control lysate
THUNDER™ EGFR Control lysate
THUNDER™ eIF2α Control lysate
THUNDER™ ERK Control lysate
THUNDER™ GAPDH Control lysate
THUNDER™ GSK3β Control lysate
THUNDER™ INR Control lysate
THUNDER™ JNK Control lysate
THUNDER™ MEK1 Control lysate
THUNDER™ MET Control lysate
THUNDER™ NF-κB Control lysate
THUNDER™ p38 Control lysate
THUNDER™ p53 Control lysate
THUNDER™ P70S6 Control lysate
THUNDER™ RPS6 Control lysate
THUNDER™ SLP-76 Control lysate
THUNDER™ SMAD2 Control lysate
THUNDER™ SRC Control lysate
THUNDER™ STAT3 Control lysate
THUNDER™ STAT5 Control lysate
THUNDER™ STAT6 Control lysate
LYSIS BUFFERS
THUNDER™ Lysis Buffer 1 (5X)
THUNDER™ Lysis Buffer 2 (5X)
THUNDER™ Lysis Buffer 3 (5X)
THUNDER™ Lysis Buffer 4 (5X)
THUNDER™ Lysis Buffer 5 (5X)
THUNDER™ Lysis Buffer 8 (5X)
THUNDER™ TR-FRET cAMP Detection Buffer (5X)
DETECTION BUFFER
THUNDER™ TR-FRET Assay Buffer 1 (5X)
THUNDER™ TR-FRET Assay Buffer 2 (5X)
THUNDER™ TR-FRET Assay Buffer 3 (5X)
THUNDER™ TR-FRET Assay Buffer 4 (5X)
THUNDER™ TR-FRET cAMP Detection Buffer (5X)
THUNDER™ TR-FRET Detection Buffer (10X)
THUNDER™ TR-FRET Kinase Assay Buffer A (5X)
THUNDER™ TR-FRET Kinase Assay Buffer B (5X)
THUNDER™ TR-FRET PPI Assay Buffer 1 (5X)
THUNDER™ TR-FRET PPI Assay Buffer 2 (5X)
THUNDER™ TR-FRET PPI Assay Buffer 3 (5X)
THUNDER™ TR-FRET PPI Assay Buffer 4 (5X)
PHOSPHATASE INHIBITOR COCKTAIL
THUNDER™ Phosphatase Inhibitor Cocktail (100X)

THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit

2019-12-12
COMPLEMENTARY REAGENTS THUNDER™ AKT pan Control lysate THUNDER™ TR-FRET Detection Buffer (10X) THUNDER™ Phosphatase Inhibitor Cocktail (100X)
View technical data sheet View generic user manual

USD$409.00 – USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT1/2/3 (S473) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available. BEST SELLER

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Related products

Stimulation of Phospho-AKT pan (S473) NIH3T3cells
Treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473.
Inhibition Phospho-AKT pan (S473) in NIH3T3 cells
Treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473.
Z'-factor determinationPhospho in NIH3T3 cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
NIH3T3 control lysate titration
Data show a linear relationship between lysate dilutions and TR FRET ratio values.
Inhibition Phospho-AKT pan (S473) in Jurkat cells
Treatment of Jurkat cells with LY294002 inhibits phosphorylation of AKT pan at S473.
Head-to-head comparison of kits
Data show that the THUNDER™ assay kit outperforms the other two competing TR-FRET kits.
Bioauxilium specializes in the design, development and manufacturing of ready-to-use TR-FRET assay kits that simplify laboratory workflow and accelerate drug discovery and life sciences research.
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