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THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT1/2/3 (S473) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available. BEST SELLER


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.
Stimulation of Phospho-AKT pan (S473) NIH3T3cells
Treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473.
Inhibition Phospho-AKT pan (S473) in NIH3T3 cells
Treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473.
Z'-factor determinationPhospho in NIH3T3 cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
NIH3T3 control lysate titration
Data show a linear relationship between lysate dilutions and TR FRET ratio values.
Inhibition Phospho-AKT pan (S473) in Jurkat cells
Treatment of Jurkat cells with LY294002 inhibits phosphorylation of AKT pan at S473.
Head-to-head comparison of kits
Data show that the THUNDER™ assay kit outperforms the other two competing TR-FRET kits.