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THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit



Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT pan (S473) in cell lysates


BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.
Stimulation of Phospho-AKT pan (S473) NIH3T3cells
Treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473.
Inhibition Phospho-AKT pan (S473) in NIH3T3 cells
Treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473.
Z'-factor determinationPhospho in NIH3T3 cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
NIH3T3 control lysate titration
Data show a linear relationship between lysate dilutions and TR FRET ratio values.
Head-to-head comparison of kits
Data show that the THUNDER™ assay kit outperforms the other two competing TR-FRET kits.