THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit

Name: THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit
SKU: 188
Availability: InStock

2019-12-12

$335.00 – $7,000.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT pan (S473) in cell lysates

N/A

Category: AKT pan

Treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473.

Treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473.

The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR FRET ratio values.

THUNDER™
TR-FRET