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THUNDER™ Phospho-eIF2α (S51) + Total eIF2α TR-FRET Cell Signaling Assay Kit

2019-12-12

$1,195.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EIF2α (S51) and Total eIF2α in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total eIF2α proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.
Stimulation of Phospho-eIF2a (S51) in Hela cells
Data show that treatment of HeLa cells with Calyculin A stimulates phosphorylation of eIF2α at S51 but does not affect the levels of total eIF2α.
Hela control lysate-titration (QC Test) PHOSPHO-eIF2α (S51)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Hela control lysate titration (QC Test) TOTAL-eIF2a
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.