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Phospho-GSK3β (S9)

2019-12-13

$335.00$1,057.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-GSK3β (S9)

N/A

Category:
Clear
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information

Size

100 points, 500 points

Stimulation of Phospho-GSK3b (S9) in MCF7 cells
Data show that treatment of MCF7 cells with Insulin stimulates phosphorylation of GSK3β at S9.
Inhibition of Phospho-GSK3b (S9) in MCF7 cells
Data show that treatment of MCF7 cells with UCN-01 inhibits phosphorylation of GSK3β at S9 by Insulin.
Z'-factor determination in MCF7 cells
The Z’-factor value was determined using a total of 48 wells for each treatment group. The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
MCF7 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.