Data show that treatment of MCF7 cells with Insulin stimulates phosphorylation of GSK3β at S9.
Data show that treatment of MCF7 cells with UCN-01 inhibits phosphorylation of GSK3β at S9 by Insulin.
The Z’-factor value was determined using a total of 48 wells for each treatment group. The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
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