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THUNDER™ Human IL-6 TR-FRET Biomarker Assay Kit

2021-02-16

$404.00$6,862.00

Sandwich TR-FRET immunoassay for the quantitative measurement of human IL-6 in cell culture supernatants. The kit does not include a recombinant human IL-6 standard, which must be purchased separately from ProSpec (catalog number CYT-213). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet. BEST SELLER

Key features and benefits:

  • Homogeneous: no wash, easy and rapid protocol
  • High sensitivity (15 µL sample), specificity, robustness, and reproducibility
  • High-quality antibody pairs ensure lot-to-lot consistency
  • Reliable: rigorous development, validation, and quality control testing
  • Cost-effective: 5 flexible formats; larger quantities available upon request
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Typical standard curve lamp
Standard curve for the THUNDER human IL-6 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using lamp excitation.
Typical standard curve laser
Standard curve for the THUNDER human IL-6 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using laser excitation.
THUNDER Human IL-6 standard curve
A head-to-head benchmark comparison of the THUNDER Human IL-6 assay (red curve above) and a competitor TR-FRET Human IL-6 assay (blue curve on the right) was conducted. Standard curves were run using the corresponding reagents and protocols.
Competitor Human IL-6 standard curve
The THUNDER assay exhibits a wider dynamic range and a much higher S/B ratio compared to the Competitor assay. In addition, the THUNDER assay generates data faster (after 1 hour of incubation versus 2 hours).
Parallelism with PBMC cells
Supernatants from PBMC cells treated for two days with LPS and IL-2 were serially diluted with culture medium and assayed for IL-6. Recovery values between 95-114% of the neat sample demonstrate assay parallelism.