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THUNDER™ Phospho-MEK1 (S218/S222) TR-FRET Cell Signaling Assay Kit

2019-12-13

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-MEK1 (S218/S222) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

N/A

Category:
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-MEK1 (S218-S222) in HeLa cells
Data show that treatment of HeLa cells with PMA stimulates phosphorylation of MEK1 at S218/S222
Inhibition of Phospho-MEK1 (S218-S222) in HeLa cells
Data show that treatment of HeLa cells with L779,450 inhibits phosphorylation of MEK1 at S218/S222 by PMA.
Z'-factor determination in HeLa cells
The Z’-factor value of 0.76 indicates that the assay is robust and suitable for HTS.
Hela control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
All-in-one-well assay in HeLa cells
Data show that stimulation of phospho-MEK1 (S218/S222) in HeLa cells can be detected using the 1-plate assay protocol.