THUNDER™ Phospho-MEK1 (S218/S222) TR-FRET Cell Signaling Assay Kit

Name: THUNDER™ Phospho-MEK1 (S218/S222) TR-FRET Cell Signaling Assay Kit
SKU: 266
Availability: InStock

2019-12-13

$355.00 – $7,315.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-MEK1 (S218/S222) in cell lysates

N/A

Category: MEK1

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Data show that treatment of HeLa cells with PMA stimulates phosphorylation of MEK1 at S218/S222

Data show that treatment of HeLa cells with L779,450 inhibits phosphorylation of MEK1 at S218/S222 by PMA.

The Z’-factor value of 0.76 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

Data show that stimulation of phospho-MEK1 (S218/S222) in HeLa cells can be detected using the 1-plate assay protocol.