Data show that treatment of HeLa cells with PMA stimulates phosphorylation of MEK1 at S218/S222
Data show that treatment of HeLa cells with L779,450 inhibits phosphorylation of MEK1 at S218/S222 by PMA.
The Z’-factor value of 0.76 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
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