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THUNDER™ Total SLP-76 TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total SLP-76 (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-SLP-76 (S376) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-SLP76T-100 Category:

Components
Eu-total-SLP76 (5 µL)
Acc-total-SLP76 (20 µL)
SLP76 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-SLP-76 (S376) in Jurkat cells
Data show that treatment of Jurkat cells with H2O2 stimulates phosphorylation of SLP-76 at S376 but does not affect the levels of total SLP-76
Jurkat control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values