Products menu

THUNDER™ Total EGFR TR-FRET Cell Signaling Assay Kit

2019-12-12

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total EGFR (Y1068) in cell lysates. This kit can be used to normalize data obtained with the Phospho-EGFR (Y1068) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

N/A

Category:
BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of A431 cells with EGF stimulates phosphorylation of EGFR at Y1068, but does not have a major effect on the levels of total EGFR.
Inhibition of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of HEK293 cells with Cetuximab inhibits phosphorylation of EGFR at Y1068 by EGF, but does not have a major effect on the levels of total EGFR.
A431 control lysate titration (QC Test)
Lysates were serially diluted with 1X Lysis Buffer 1 and tested in triplicate