Name: THUNDER™ High Performance Phospho-STAT1 (Y701) TR-FRET Cell Signaling Assay Kit
SKU: 4002
Availability: InStock

THUNDER™ High Performance Phospho-STAT1 (Y701) TR-FRET Cell Signaling Assay Kit

2024-07-09

View technical data sheet View generic user manual

USD$430.00 – USD$8,809.00

High performance sandwich TR-FRET immunoassay for semi-quantitative detection of Human and Mouse Phospho-STAT1 (Y701) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: This kit is a new and improved high performance version of catalog number KIT-STAT1P-XXX, which has been discontinued.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

SKU: KIT-HP-STAT1P-100 Categories: STAT1, TR-FRET CELL SIGNALING ASSAY KITS

Size

Clear

Components
Eu-HP-phospho-STAT1 (5 µL)
Acc-HP-phospho-STAT1 (20 µL)
STAT1 control lysate (100 µL)
5X Lysis Buffer 8 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human, Mouse

USD$430.00

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

High Performance Phospho-STAT1 vs Current Kit

Data show the superior S/B ratio obtained with the High Performance Phospho-STAT1 (Y701) Kit compared to the standard version of the kit.

High Performance Phospho-STAT1 stimulation in HeLa cells

Data show that treatment of HeLa cells with IFNα2b stimulates phosphorylation of STAT1 at Y701. Robust S/B ratio and pharmacology data are obtained after 1h of incubation.

High Performance Phospho-STAT1 inhibition in HeLa cells

Data show that treatment of HeLa with JAK Inhibitor 1 inhibits phosphorylation of STAT1 at Y701 by IFNα2b. Robust S/B ratio and pharmacology data are obtained after 1h of incubation.

High Performance Phospho-STAT1 - Z'-factor

The Z’-factor value of 0.89 indicates that the assay is robust and suitable for HTS.

High Performance Phospho-STAT1 in NIH/3T3

Data show that the High Performance kit detects mouse Phospho-STAT1 (Y701).

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ High Performance Phospho-STAT1 (Y701) TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ High Performance Phospho-STAT1 (Y701) TR-FRET Cell Signaling Assay Kit

Additional information

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THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS