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THUNDER® Total CDK6 TR-FRET Cell Signaling Assay Kit

2026-05-15

Price range: USD$409.00 through USD$8,390.00

Sandwich TR-FRET immunoassay designed for the semi-quantitative detection of Human Total CDK6 (Cyclin-Dependent Kinase 6; UniProt ID: Q00534; Entrez-Gene Id: 1021) in cell lysates. The assay uses the advanced THUNDER® TR-FRET technology and a matched antibody pair specifically optimized and validated for TR-FRET. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. The assay is compatible with both adherent and suspension cells and can be run under either a one-plate or two-plate protocol. Both methods are homogeneous (no-wash).

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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Degradation of Total CDK6 by YX-2-107 in Jurkat cells
Data show that treatment of Jurkat cells with the CDK6-degrading PROTAC YX-2-107 degrades total CDK6 in a concentration-dependent manner. In contrast, CDK6 levels are not modulated by PROTAC CDK2 Degrader CPS2.
Time course of CDK6 detection
Data show that robust S/B ratio and pharmacology can be obtained after just 1h of incubation.
Z'-factor of CDK6 assay with Jurkat cells
The Z'-factor of 0.86 indicates that the assay is robust and suitable for HTS.
CDK6 assay specificity
Data show that the CDK2 assay specifically detects CDK6 without any cross-reactivity toward the other CDKs tested.
QC test-CDK6
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.