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THUNDER™ Phospho-SMAD3 (S423/S425) + Total SMAD3 TR-FRET Cell Signaling Assay Kit

2025-06-13

USD$1,283.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD3 (S423/S425) and Total SMAD3 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total SMAD3 proteins in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with TGFβ stimulates phosphorylation of SMAD3 at S423/S425, but does not have an effect on the levels of total SMAD3.
Inhibition of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with SB 431542 inhibits phosphorylation of SMAD3 at S423/S425 by TGFβ1, but does not have an effect on the levels of total SMAD3.
A549 control lysate titration (QC Test)-Phospho-SMAD3
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
A549 control lysate titration (QC Test)-Total SMAD3
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.