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THUNDER™ Phospho-STAT2 (Y690) TR-FRET Cell Signaling Assay Kit

2024-10-29

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT2 (Y690) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-STAT2P-100 Categories: ,

Components
Eu-phospho-STAT2 (5 µL)
Acc-phospho-STAT2 (20 µL)
STAT2 control lysate (100 µL)
5X Lysis Buffer 1 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human, Mouse

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation-Phospho-STAT2
Data show that treatment of A431 cells with IFNα2b stimulates phosphorylation of STAT2 at Y690.
Inhibition-Phospho-STAT2
Data show that treatment of A431 cells with JAK Inhibitor 1 inhibits phosphorylation of STAT2 at Y690 by IFNα2b.
Z-factor-Phospho-STAT2
The Z’-factor value of 0.86 indicates that the assay is robust and suitable for HTS
A431 control lysate titration
Data show a linear relationship between lysate dilutions and TR-FRET ratio value.