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THUNDER™ Total RIPK1 TR-FRET Cell Signaling Assay Kit

2024-07-17

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total RIPK1 (both phosphorylated and non-phosphorylated RIPK1) in cell lysates. This kit can be used to normalize data obtained with the Phospho-RIPK1 (S166) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-RIPK1T-100 Categories: ,

Components
Eu-total-RIPK1 (5 µL)
Acc-total-RIPK1 (20 µL)
RIPK1 control lysate (100 µL)
5X Lysis Buffer 1 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Inhibition of Phospho-RIPK1 in HT-29 cells
Data show that treatment of HT-29 cells with Necrostatin-1S inhibits phosphorylation of RIPK1 at S166 by TNF-alpha (in the presence of SM-164 and Z-VAD) but does not have a major effect on the levels of total RIPK1.
HT-29 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.