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THUNDER™ Phospho-STAT6 (Y641) TR-FRET Cell Signaling Assay Kit

2019-12-13

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT6 (Y641) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Product citation: PubMed 34570089

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BioAuxilium sells direct to Canada- and US-based customers only. International clients can purchase through one of our
international distributors.

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Stimulation of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with IL-4 stimulates phosphorylation of STAT6 at Y641, but does not affect the levels of total STAT6
Inhibition of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4, but does not affect the levels of total STAT6
HeLa control lysate titration (QC Test) Phospho-STAT6 (Y641
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
HeLa control lysate titration (QC Test) Total STAT6
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
All-in-one-well Phospho-STAT6 (Y641) assay
Data show that stimulation of phospho-STAT6 at Y641 can be detected using the 1-plate (all-in-one-well) assay protocol.