Products menu

THUNDER™ Phospho-STAT6 (Y641) TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT6 (Y641) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

Note: a new and improved version of this kit is available: High Performance Phospho-STAT63 (Y641) Assay Kit

Product citation: PubMed 34570089

SKU: KIT-STAT6P-5000 Category:

Components
Eu-phospho-STAT6 (250 µL)
Acc-phospho-STAT6 (1 mL)
STAT6 control lysate (2 x 200 µL)
5X Lysis Buffer 2 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)

Reactivity
Human, Mouse

USD$6,763.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with IL-4 stimulates phosphorylation of STAT6 at Y641, but does not affect the levels of total STAT6
Inhibition of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4, but does not affect the levels of total STAT6
HeLa control lysate titration (QC Test) Phospho-STAT6 (Y641
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
HeLa control lysate titration (QC Test) Total STAT6
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
All-in-one-well Phospho-STAT6 (Y641) assay
Data show that stimulation of phospho-STAT6 at Y641 can be detected using the 1-plate (all-in-one-well) assay protocol.