THUNDER™ Total STAT5 TR-FRET Cell Signaling Assay Kit

2019-12-13

View technical data sheet View generic user manual

$409.00 – $8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total STAT5 (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-STAT5 (Y694/Y699) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product citation: PubMed 34570089

SKU: N/A Category: STAT5

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Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-STAT5 (Y694/699) in A431 cells

Data show that treatment of A431 cells with EGF stimulates phosphorylation of STAT5 at Y694/Y699, but does not have a major effect on the levels of total STAT5

Inhibition of Phospho-STAT5 (Y694/699) in A431 cells

Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of STAT5 at Y694/Y699 by EGF, but does not have a major effect on the levels of total STAT5

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Total STAT5 TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Total STAT5 TR-FRET Cell Signaling Assay Kit

Additional information

Related products

THUNDER™ Phospho-STAT5 (Y694/Y699) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-STAT5 (Y694/Y699) + Total STAT5 TR-FRET Cell Signaling Assay Kit

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS