Products menu

THUNDER™ Total STAT5 TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total STAT5 (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-STAT5 (Y694/Y699) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product citation: PubMed 34570089

SKU: KIT-STAT5T-100 Category:

Components
Eu-Total STAT5 (5 µL)
Acc-Total STAT5 (20 µL)
STAT5 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human, Mouse

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-STAT5 (Y694/699) in A431 cells
Data show that treatment of A431 cells with EGF stimulates phosphorylation of STAT5 at Y694/Y699, but does not have a major effect on the levels of total STAT5
Inhibition of Phospho-STAT5 (Y694/699) in A431 cells
Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of STAT5 at Y694/Y699 by EGF, but does not have a major effect on the levels of total STAT5
A431 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values